Protein_Domain

Part:BBa_K4638021:Design

Designed by: Hao Yu   Group: iGEM23_PekingHSC   (2023-10-11)


EX-HA-MUT


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1436
    Illegal SpeI site found at 1959
    Illegal PstI site found at 1877
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1436
    Illegal SpeI site found at 1959
    Illegal PstI site found at 1877
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1436
    Illegal BglII site found at 532
    Illegal XhoI site found at 311
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1436
    Illegal SpeI site found at 1959
    Illegal PstI site found at 1877
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1436
    Illegal SpeI site found at 1959
    Illegal PstI site found at 1877
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Influenza should be carefully engineered. If you want to use this composite part in other types of Influenza, you need to do the same way of mutation in both the protein and the packaging signal so that the virus can be successfully packaged.


Source

Extracted from the DNA sequence of WSN from NIH.

References