![](https://parts.igem.org/images/partbypart/icon_protein_domain.png)
Protein_Domain
Part:BBa_K4638021:Design
Designed by: Hao Yu Group: iGEM23_PekingHSC (2023-10-11)
EX-HA-MUT
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1436
Illegal SpeI site found at 1959
Illegal PstI site found at 1877 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1436
Illegal SpeI site found at 1959
Illegal PstI site found at 1877 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1436
Illegal BglII site found at 532
Illegal XhoI site found at 311 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1436
Illegal SpeI site found at 1959
Illegal PstI site found at 1877 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1436
Illegal SpeI site found at 1959
Illegal PstI site found at 1877 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Influenza should be carefully engineered. If you want to use this composite part in other types of Influenza, you need to do the same way of mutation in both the protein and the packaging signal so that the virus can be successfully packaged.
Source
Extracted from the DNA sequence of WSN from NIH.